hybridization buffer  (GE Healthcare)

Journal: The Journal of Neuroscience

Article Title: Single-Cell Microarray Analysis in Hippocampus CA1: Demonstration and Validation of Cellular Heterogeneity

doi: 10.1523/JNEUROSCI.23-09-03607.2003

Figure Lengend Snippet: Partial microarray image. The microarray was hybridized to a Cy3-labeled target generated from a single cell. The identities of seven genes are highlighted.

Article Snippet: The entire generated Cy3-labeled target was hybridized overnight at 42°C onto a single chip in a buffer containing 50% formamide and 1× Microarray Hybridization buffer (RPK-0325, Amersham Biosciences).

Techniques: Microarray, Labeling, Generated

Journal: The Journal of Neuroscience

Article Title: Single-Cell Microarray Analysis in Hippocampus CA1: Demonstration and Validation of Cellular Heterogeneity

doi: 10.1523/JNEUROSCI.23-09-03607.2003

Figure Lengend Snippet: Estimation of the sensitivity of the combination of laser capture microdissection, T7 aRNA amplification, and cDNA microarray analysis using quantitative RT-PCR

Article Snippet: The entire generated Cy3-labeled target was hybridized overnight at 42°C onto a single chip in a buffer containing 50% formamide and 1× Microarray Hybridization buffer (RPK-0325, Amersham Biosciences).

Techniques: Laser Capture Microdissection, Amplification, Microarray

Journal: The Journal of Neuroscience

Article Title: Single-Cell Microarray Analysis in Hippocampus CA1: Demonstration and Validation of Cellular Heterogeneity

doi: 10.1523/JNEUROSCI.23-09-03607.2003

Figure Lengend Snippet: Hierarchical clustering of the microarray data set. Each column represents a single cell, each row a gene. Three clusters are indicated by an orange box to the left of the columns and by roman numerals I–III to the right of the columns. These three clusters are shown in Figure ​Figure55.

Article Snippet: The entire generated Cy3-labeled target was hybridized overnight at 42°C onto a single chip in a buffer containing 50% formamide and 1× Microarray Hybridization buffer (RPK-0325, Amersham Biosciences).

Techniques: Microarray

Journal: The Journal of Neuroscience

Article Title: Single-Cell Microarray Analysis in Hippocampus CA1: Demonstration and Validation of Cellular Heterogeneity

doi: 10.1523/JNEUROSCI.23-09-03607.2003

Figure Lengend Snippet: Microarray data validation using double-labelingin situ hybridization. Five genes selected from two of the cluster described in Figure ​Figure55 were analyzed by in situ hybridization together with parvalbumin. Presence of parvalbumin mRNA is indicated by light-gray staining, and the colocalizing gene is indicated by black silver grains. The right column shows a nuclear counterstain, using YO-PRO, of the same field of view as the respective in situ hybridization. Scale bar, 50 μm.

Article Snippet: The entire generated Cy3-labeled target was hybridized overnight at 42°C onto a single chip in a buffer containing 50% formamide and 1× Microarray Hybridization buffer (RPK-0325, Amersham Biosciences).

Techniques: Microarray, Hybridization, In Situ Hybridization, Staining